Experimental models |
- Microdialysis and Biosensors - Disease models - from rodents to NHPs - Microsurgery, stereotaxic surgery - Pharmacokinetics and drug brain delivery |
Applications of microdialysis in preclinical research and drug discovery require a unique combination of highly specialized skills in microsurgery, stereotaxic surgery and in vivo handling protocols for small rodents, as well as, knowledge in neuroanatomy and pharmacology of major neurotransmitter systems.
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Microdialysis protocols on anesthetized or awake rats and mice "Proof-of-principle" studies, monitoring neurotransmitters and other surrogate markers, PK/PD studies, drug delivery through BBB, targeted bioavailability. All microdialysis experiments carried out with simultaneous recordings of locomotor activity. |
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Disease models Behavioral tests in rodent (rat, mouse, transgenic animals) models of psychiatric, neurodegenerative and metabolic disorders: Schizophrenia, depression, ADHD, drug abuse liability, anxiety, learning and memory dysfunction, epilepsy, Parkinson´s disease, Alzheimer´s disease, Huntington´s disease, traumatic brain injury, liver encephalopathy, diabetes, immunodeficient models. |
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Microsurgery, stereotaxic surgery on small rodents Microsurgery at aseptic conditions. Intraventricular, intravenous injection/infusion cannulae including microdialysis probes, syringe minipump-based continuous/discontinuous drug infusions for up to 4 weeks, Alzet osmotic pumps. Sampling CSF. |
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Lesion models of neurodegenerative diseases. 6-OHDA, MPTP-lesion models of Parkinson´s disease. 192IgG-saporin lesions of cholinergic neurons. Ibotenic acid lesions. |
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Translational capabilities In partnership with Preclinical Research Center, Lexington, University of Kentucky, we offer PK/PD studies on NHPs to be designed on a unified experimental and bioanalytical platform. |
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Pharmacokinetics and drug brain delivery Dual- or Triple microdialysis - PK profiles of unbound drug concentrations in microdialysates, blood and brain compartments. Targeted drug delivery through the BBB. LC-MS/MS method development and validation for measuring drugs and test compounds. PD profiles - effects of drugs on neurotransmitter release, uptake and metabolism. |