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Bioanalytical services

Liquid chromatography
- Mass spectrometry (LC-MS)

- HPLC with electrochemical detection (LCEC,

  amperomety, coulometry)

- HPLC with fluorescence detection

- HPLC with UV/VIS (DAD) detection

- HPLC with conductivity detection
Immunoassays
- EIA, ELISA with UV/VIS, FL, TRF and LM detection
Enzyme assays
- Enzyme activity

- Biomarkers including glucose, lactate, pyruvate,

  glycerol
Spectrophotometric assays - Inorganic ions, calcium, magnesium, urea
Method development and validation
- LC-MS/MS for measuring unusual endogenous

  molecules, drugs and metabolites


The experimental armamentarium of Pronexus instrumentation covers the techniques for high-sensitive analysis of all classic neurotransmitters, their precursors and metabolites, neuromodulators, trophic factors and other neurochemically active molecules identified as surrogate markers of psychiatric, neurodegenerative, neurological and metabolic disorders. In addition, we develop and validate methods for measuring drug levels in all typical biological matrices including blood, CSF, urine, tissue, cell lysates, superfusates, hair and nails.

  UHPLC-MS/MS and UPLC-TOF systems for small and large molecules
Techniques applying ultra high performance liquid chromatography with tandem mass spectrometry and time-of-flight mass spectrometry allow detection of endogenous molecules, as well as, test compounds, drugs used as positive controls and their metabolites in biological samples including microdialysates, cell lysates, CSF, plasma and brain tissue extracts.
 
HPLC techniques with electrochemical or fluorescence detection optimized for analysis of neurotransmitters, neuromodulators and related molecules in the microdialysates
Serotonin, noradrenaline, dopamine and their metabolites, acetylcholine, GABA, aspartate and glutamate, glycine, physiological amino acids, histamine, adenosine and other purines, cAMP, cGMP, kynurenic acid and other metabolites of the kynurenine pathway, radical oxygen species (ROS), NO2-/NO3-.
 
ELISA and enzymatic spectrophotometric assays
Neuropeptides, hormones, A-beta and tau peptides, synuclein, cytokines, BDNF, NGF, interleukins enzymes and other clinical biomarkers.
Lactate, pyruvate, glycerol, glucose, urea (CMA Microdialysis Analyser) for clinical microdialysates, Mg2+, Ca2+ and other ions..

Ultra-sensitive detection of monoamines
Unique fluorescence derivatization/HPLC methods developed for simultaneous determination of noradrenaline, dopamine and serotonin down to 10 -11 M [100 atmol/20 µl]  and for histamine [300 atmol/20 µl].